Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Instrument Calibration01:12

Instrument Calibration

Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spot on: A Laser Micromachining-Based Approach to Improve Dried Matrix Spot Preparation with Proof-of-Principle Analytical Demonstrations Using Ambient Ionization Mass Spectrometry.

Micromachines·2026
Same author

Preventing chick culling in the poultry industry with a new biomarker for rapid in ovo gender screening.

Scientific reports·2026
Same author

The product of NH<sub>3</sub> loss from gas phase protonated tyrosine.

Physical chemistry chemical physics : PCCP·2026
Same author

Ultra-high throughput mass spectrometry with ultra high-speed separations: differential mobility spectrometry-acoustic ejection mass spectrometry (DAEMS).

Bioanalysis·2025
Same author

High-Throughput Detection and Quantitation of Metal Contamination with Acoustic Ejection Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2025
Same author

A Differential Ion Mobility Acoustic Ejection Mass Spectrometer System for Screening Isomerization-Mediating Enzyme Drug Targets.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jul 16, 2026

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) Mass Spectrometry
06:56

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) Mass Spectrometry

Published on: June 10, 2018

Calibrant delivery for mass spectrometry.

Bradley B Schneider1, Thomas R Covey

  • 1MDS SCIEX, Concord, Ontario, Canada. bradley.schneider@sciex.com

Journal of the American Society for Mass Spectrometry
|March 27, 2007
PubMed
Summary

This study introduces a novel method for calibrating mass spectral analysis by introducing calibrant ions remotely, avoiding interference and improving accuracy. This technique enhances external calibration without compromising primary ion source performance.

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Traditional mass spectral analysis calibration methods often face challenges with ion source interference and performance compromises.
  • Dual ion sources can lead to detrimental interactions between gas flows and electric fields.

Purpose of the Study:

  • To develop a new method for on-demand delivery of calibrant ions in mass spectral analysis.
  • To enable external calibration that avoids complications associated with conventional dual-source setups.
  • To allow for simultaneous sampling of multiple ion beams for internal mass calibration.

Main Methods:

  • Utilizing a remote ion source for calibrant ions within an atmospheric pressure chamber.
  • Employing a gas flow to direct calibrant ions towards the sampling inlet.

More Related Videos

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

MALDI Sample Preparation: the Ultra Thin Layer Method
05:28

MALDI Sample Preparation: the Ultra Thin Layer Method

Published on: April 29, 2007

Related Experiment Videos

Last Updated: Jul 16, 2026

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) Mass Spectrometry
06:56

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) Mass Spectrometry

Published on: June 10, 2018

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

MALDI Sample Preparation: the Ultra Thin Layer Method
05:28

MALDI Sample Preparation: the Ultra Thin Layer Method

Published on: April 29, 2007

  • Implementing a repelling electrode field to control the toggling of ion beams into the instrument.
  • Modulating the electrical potential applied to a primary nanoflow electrospray tip to control calibrant ion sampling.

    • Main Results:

    • Physical separation of ion sources eliminates detrimental interactions.
    • External calibration is achieved without compromising primary electrospray performance.
    • The method allows for on-demand sampling of calibrant ions.
    • Successful application with atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) and different ion polarities.

    Conclusions:

    • This novel approach provides a robust and versatile method for mass spectral calibration.
    • It overcomes limitations of existing dual-source calibration techniques.
    • The technique is adaptable to various atmospheric pressure ionization sources and ion polarities.