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Updated: Jul 16, 2026

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
Published on: October 23, 2018
[Energy-proteolysis and regulation of protein metabolism]
Abstract:
Energy-proteolysis is the integral part of cell protein turnover Proteolysis controls the quality of protein post-translational modification, folding as well as the structural and functional cell activities. Several proteolytic systems including 26S proteasomes participate in the cell protein degradation. Human diet regulates the rate of protein turnover. Total protein level, amino acid composition, fasting, toxins, etc. modify the receptor expression, signal transduction, factor transcription activity and expression of genes to regulate the cellular response in metabolic stress. Activities of some ATPases are dependent on the nutritional factors. Free amino acid peptides released from proteasomes may control the protein-protein interaction and cellular metabolic pathways.
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