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Related Concept Videos

Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

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Matrix-assisted laser desorption/ionization (MALDI) mechanism revisited.

Wei Chao Chang1, Ling Chu Lora Huang, Yi-Sheng Wang

  • 1Genomics Research Center, Academia Sinica, Taipei, Taiwan.

Analytica Chimica Acta
|March 28, 2007
PubMed
Summary

The energy transfer induced disproportionation (ETID) model explains analyte ion formation in matrix-assisted laser desorption/ionization (MALDI). This new model addresses limitations of existing photochemical ionization (PI) and cluster ionization (CI) mechanisms.

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Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) Mass Spectrometry
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Published on: June 10, 2018

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Biochemistry

Background:

  • Matrix-assisted laser desorption/ionization (MALDI) is a widely used technique for analyzing biomolecules.
  • The precise ionization mechanism in MALDI remains incompletely understood.
  • Existing photochemical ionization (PI) and cluster ionization (CI) models do not fully explain all experimental observations.

Purpose of the Study:

  • To propose a novel mechanism for analyte ion formation in MALDI.
  • To explain the simultaneous production of positive and negative ions for large biomolecules.
  • To compare the proposed model with existing MALDI ionization mechanisms.

Main Methods:

  • Development of the energy transfer induced disproportionation (ETID) model.
  • Theoretical consideration of a pseudo proton transfer process during crystallization.
  • Comparison of the ETID model with PI and CI models using experimental data.

Main Results:

  • The ETID model provides a framework for understanding analyte ion generation in MALDI.
  • The model accounts for the observation of equimolar positive and negative ion formation for large biomolecules.
  • The ETID model offers a potential explanation for phenomena not covered by PI and CI mechanisms.

Conclusions:

  • A pseudo proton transfer during crystallization is proposed as a primary ion formation mechanism in MALDI.
  • The energy transfer induced disproportionation (ETID) model offers a new perspective on MALDI ionization.
  • Further experimental validation is needed to fully establish the ETID model's applicability.