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Immobilization of cholesterol esterase and cholesterol oxidase onto sol-gel films for application to cholesterol
Suman Singh1, Rahul Singhal, B D Malhotra
1Central Mechanical Engineering Research Institute, G. Avenue, Durgapur 713209, West Bengal, India.
Abstract:
Cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) have been covalently immobilized onto tetraethylorthosilicate (TEOS) sol-gel films. The tetraethylorthosilicate sol-gel/ChEt/ChOx enzyme films thus prepared have been characterized using scanning electron microscopic (SEM), UV-vis spectroscopic, Fourier-transform-infrared (FTIR) spectroscopic and amperometric techniques, respectively. The results of photometric measurements carried out on tetraethylorthosilicate sol-gel/ChEt/ChOx reveal thermal stability up to 55 degrees C, response time as 180 s, linearity up to 780 mg dL(-1) (12 mM), shelf life of 1 month, detection limit of 12 mg dL(-1) and sensitivity as 5.4 x 10(-5) Abs. mg(-1) dL(-1).
