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Published on: August 16, 2019
Development and application of immunoaffinity column chromatography for atrazine in complex sample media
Jane C Chuang1, Jeanette M Van Emon, Randy Jones
1Battelle, Columbus, OH, USA. chuangj@battelle.org
Analytica Chimica Acta
|March 28, 2007
Summary
This study introduces an immunoaffinity (IA) column cleanup method for atrazine determination. The IA columns effectively remove interferences from soil, sediment, and food samples, enabling accurate analysis.
Area of Science:
- Analytical Chemistry
- Environmental Science
Background:
- Atrazine is a widely used herbicide, and its accurate determination in environmental and food samples is crucial.
- Existing methods for atrazine analysis can be complex and time-consuming, often requiring extensive sample cleanup.
Purpose of the Study:
- To evaluate an immunoaffinity (IA) column procedure as an efficient cleanup method for atrazine determination.
- To assess the performance of IA columns in complex matrices like soil, sediment, and food.
Main Methods:
- Development and validation of rabbit antibody immunoaffinity (IA) columns using HiTrap Sepharose.
- Testing of various loading and elution solvents for optimal atrazine binding and release.
- Evaluation of IA column performance with spiked soil, sediment, and food samples.
- Comparison of IA column-assisted analysis with gas chromatography/mass spectrometry (GC/MS) and enzyme-linked immunosorbent assay (ELISA).
Main Results:
- IA columns demonstrated reproducible antibody immobilization and consistent atrazine binding and release.
- High coupling efficiency (96+/-2%) and good recoveries (107-122%) for atrazine standards.
- IA columns effectively removed matrix interferences from soil, sediment, and food samples.
- IA columns could be reused over 50 times without performance degradation.
- Significant correlation between ELISA and GC/MS data, with IA-ELISA showing promise as an alternative method.
Conclusions:
- The immunoaffinity (IA) column procedure is a robust and effective cleanup method for atrazine determination.
- This IA column method simplifies sample preparation and enhances analytical accuracy in complex matrices.
- IA column coupled with ELISA offers a viable alternative analytical approach for atrazine monitoring.
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