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Related Experiment Video

Updated: Jul 16, 2026

Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril
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Simultaneous detection of ascorbate and uric acid using a selectively catalytic surface.

Hossam M Nassef1, Abd-Elgawad Radi, Ciara O'Sullivan

  • 1Department of Chemical Engineering, University of Rovira I Virgili, Av.Paisos Catalan, 26, 43007 Tarragona, Spain.

Analytica Chimica Acta
|March 28, 2007
PubMed
Summary

Researchers developed a modified glassy carbon electrode (GCE) for sensitive ascorbate detection. This new electrode offers improved electrocatalytic activity and selectivity, enabling accurate quantification in real samples.

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Area of Science:

  • Electrochemistry
  • Analytical Chemistry
  • Materials Science

Background:

  • Direct detection of ascorbate is challenging due to high overpotential and electrode fouling.
  • Electrode modification using electrochemical reduction of diazonium salts offers catalytic and analytical advantages.

Purpose of the Study:

  • To develop a modified glassy carbon electrode (GCE) for selective and direct detection of ascorbate.
  • To investigate the electrocatalytic properties of the modified electrode for ascorbate oxidation.
  • To enable simultaneous determination of ascorbate and uric acid in real samples.

Main Methods:

  • Grafting a monolayer of o-aminophenol (o-AP) onto a GCE via electrochemical reduction of diazonium salts.
  • Characterization of the modified surface using cyclic voltammetry.

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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
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Last Updated: Jul 16, 2026

Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril
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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

  • Quantitative determination of ascorbate and uric acid using cyclic voltammetry and d.c. amperometry.

    • Main Results:

    • The o-AP modified GCE exhibited excellent electrocatalytic activity, reducing ascorbate oxidation overpotential from +462 mV to +263 mV.
    • A linear calibration curve for ascorbate was obtained in the range of 2-20 microM with a limit of detection of 0.3 microM.
    • The modified electrode demonstrated high stability and was successfully applied for simultaneous detection of ascorbate and uric acid in real urine samples.

    Conclusions:

    • The o-aminophenol modified GCE (o-AP/GCE) provides a simple, precise, and stable amperometric sensor for selective ascorbate determination.
    • The developed electrode facilitates efficient electrocatalytic oxidation of ascorbate, overcoming limitations of conventional electrodes.
    • This method is suitable for the simultaneous analysis of ascorbate and uric acid in complex biological matrices like urine.