Related Experiment Video
Updated: Jul 16, 2026
Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
Published on: March 9, 2018
Changes in the elastase activity and colonization ability of Aspergillus fumigatus after successive inoculations in
Marta E García1, Jesús Caballero, Isabel Blanco
1Departamento Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Abstract:
In a previous work we demonstrated a clear link between elastase activity and pathogenicity using what we have named the Elastase Activity Index (EAI). In the present study we have evaluated the possible variability of this index as a consequence of successive inoculations in mice. Two strains of Aspergillus fumigatus isolated from the environment without elastase activity were used. These strains were inoculated into successive batches of ten mice. Our results showed that with each inoculation there was an increase in the number of mice on each batch from which the strain could be isolated and an increase in the number of strains with an EAI>1. This study suggests that A. fumigatus could adapt to the environment in which it is developed, increasing its pathogenic capabilities from host to host.
Insights
Aspergillus fumigatus strains adapted to hosts, increasing pathogenicity with each mouse inoculation. This suggests airborne fungal pathogens can evolve enhanced virulence through host-to-host transmission.
Area of Science:
- Medical Mycology
- Pathogen Adaptation
- Infectious Disease Dynamics
Background:
- Previous research established a link between elastase activity and pathogenicity using the Elastase Activity Index (EAI).
- The variability of EAI in Aspergillus fumigatus following successive host passages was not previously understood.
Purpose of the Study:
- To investigate the adaptability of Aspergillus fumigatus strains.
- To determine if successive inoculations in mice alter the elastase activity and pathogenicity of A. fumigatus.
Main Methods:
- Two non-elastolytic Aspergillus fumigatus strains were utilized.
- These strains were serially inoculated into successive batches of ten mice.
- Elastase Activity Index (EAI) was measured for isolated strains.
Main Results:
- With each successive inoculation, an increased number of mice yielded the A. fumigatus strain.
- A growing proportion of isolated strains exhibited an EAI greater than 1.
- This indicates a stepwise increase in elastase activity and potential pathogenicity.
Conclusions:
- Aspergillus fumigatus demonstrates adaptive capabilities in response to host environments.
- Serial host passage can enhance the pathogenic potential of A. fumigatus.
- Findings suggest a mechanism for increased virulence in fungal pathogens through host-to-host transmission.
More Related Videos
15:01Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
Published on: September 18, 2021
06:43Studying Microbial Communities In Vivo: A Model of Host-mediated Interaction Between Candida Albicans and Pseudomonas Aeruginosa in the Airways
Published on: January 13, 2016