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Related Experiment Videos

DNA core ionization and cell inactivation.

Arnaud Boissière1, Christophe Champion, Alain Touati

  • 1Life Sciences Division, Lawrence Berkeley National Laboratory, California 94720, USA. Arnaud.Boissiere@GMail.com

Radiation Research
|March 29, 2007
PubMed
Summary

Core ionizations in DNA atoms are critical for cell inactivation by radiation. This study reveals a significant contribution of these events to cell death, suggesting novel mechanisms in radiation biology.

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Area of Science:

  • Radiation biology
  • Molecular mechanisms of DNA damage

Background:

  • Ionizing radiation can cause DNA damage, leading to cell inactivation.
  • The role of inner-shell ionizations (core ionizations) in DNA damage and cell death is not fully understood.

Purpose of the Study:

  • To investigate whether core ionizations in DNA atoms are critical events for cell inactivation by ionizing radiation.
  • To quantify the contribution of core ionizations to cell inactivation by energetic electrons and gamma rays.

Main Methods:

  • Monte Carlo track simulations were used to calculate the number of core ionizations in DNA per gray of radiation.
  • Experimental relative biological effectiveness (RBE) values of ultrasoft X-rays were used to deduce the probability of cell inactivation per core ionization.

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Main Results:

  • The number of core ionizations in DNA atoms per gray was calculated for 100 keV electrons and gamma rays.
  • The contribution of core ionizations in DNA to V79 cell inactivation was found to be 75% +/- 27% for these radiations.

Conclusions:

  • Core ionizations in DNA are a significant contributor to cell inactivation by energetic electrons and gamma rays.
  • The substantial contribution suggests the involvement of new mechanisms and critical lesions in radiation-induced cell death.