Related Experiment Video
Updated: Jul 16, 2026
13:14
Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
Published on: August 10, 2009
Spotted cotton oligonucleotide microarrays for gene expression analysis.
Joshua A Udall1, Lex E Flagel, Foo Cheung
1Department of Plant and Animal Sciences, Brigham Young University, Provo, UT 84062, USA. jaudall@byu.edu
BMC Genomics
|March 29, 2007
Summary
A new cotton oligonucleotide microarray is now publicly available for plant biology research. This tool enables reproducible transcription profiling across all four cultivated cotton species, aiding crop improvement.
Area of Science:
- Plant Biology
- Genomics
- Agricultural Science
Background:
- Microarrays are essential tools for plant biology and crop improvement.
- Recent cotton EST assemblies provide a foundation for new genomic tools.
- A publicly available oligonucleotide array for cotton has been developed.
Purpose of the Study:
- To design and create a publicly available oligonucleotide microarray for cotton.
- To support research across all four cultivated Gossypium species.
- To facilitate gene discovery and transcriptome analysis in cotton.
Main Methods:
- Generated synthetic oligonucleotide probes from a global assembly of 211,397 cotton ESTs.
- Included 22,787 probes targeting transcriptome diversity, known genes, and transcription factors.
- Utilized >50 cDNA libraries representing diverse tissues and treatments, including fiber-specific libraries.
Main Results:
- Developed a cotton oligonucleotide microarray with 22,787 probes.
- Probes target diverse transcripts, known genes, and include elements for gene discovery.
- Demonstrated technical consistency using hybridized cDNAs from G. hirsutum buds and leaves.
Conclusions:
- The cotton oligonucleotide microarray offers a reproducible platform for transcription profiling.
- This valuable research tool is publicly accessible via http://cottonevolution.info.
- Facilitates analysis of the cotton fiber transcriptome and gene discovery.
