Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two yeast PUF proteins negatively regulate a single mRNA.

Brad A Hook1, Aaron C Goldstrohm, Daniel J Seay

  • 1Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.

The Journal of Biological Chemistry
|March 29, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The RNA-binding activity of the <i>Drosophila</i> Brat protein is necessary for viability and mRNA regulation.

RNA biology·2026
Same author

RNA-binding is the essential biological function of the <i>Drosophila</i> protein Brat.

bioRxiv : the preprint server for biology·2026
Same author

Human pumilio proteins use fuzzy multivalent hydrophobic interactions to recruit the CCR4-NOT deadenylase complex to repress mRNAs.

The Journal of biological chemistry·2026
Same author

Cytoplasmic poly-adenosine binding proteins modulate susceptibility of mRNAs to Pumilio-mediated decay.

Nucleic acids research·2026
Same author

Human Pumilio proteins use fuzzy multivalent hydrophobic interactions to recruit the CCR4-NOT deadenylase complex to repress mRNAs.

bioRxiv : the preprint server for biology·2025
Same author

Cytoplasmic poly-adenosine binding proteins modulate susceptibility of mRNAs to RNA-binding protein-directed decay.

bioRxiv : the preprint server for biology·2025

Two PUF proteins, Puf4p and Mpt5p, regulate mRNA stability and translation in yeast by binding adjacent sites on the HO endonuclease mRNA. Their combined action is crucial for full repression and mRNA decay.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Post-transcriptional Control

Background:

  • Protein repressors binding to 3'-untranslated regions (3'-UTRs) control mRNA stability and translation.
  • PUF proteins are key repressors, inhibiting translation, enhancing mRNA decay, and promoting poly(A) removal.

Purpose of the Study:

  • To investigate the regulation of a single mRNA, Saccharomyces cerevisiae HO endonuclease mRNA, by two distinct PUF proteins.
  • To elucidate the mechanisms and combinatorial action of Puf4p and Mpt5p in mRNA regulation.

Main Methods:

  • In vivo analysis of mRNA stability and deadenylation in Saccharomyces cerevisiae.
  • Investigating the binding and co-occupancy of Puf4p and Mpt5p on the target mRNA.
  • Assessing the repression mechanisms dependent on deadenylation.

Related Experiment Videos

Main Results:

  • Puf4p and Mpt5p bind to adjacent sites on the HO endonuclease mRNA and can co-occupy it.
  • Both proteins are essential for complete repression and deadenylation; their absence stabilizes the mRNA.
  • Puf4p repression is deadenylation-dependent, while Mpt5p utilizes additional repression mechanisms.

Conclusions:

  • Combinatorial action of Puf4p and Mpt5p provides robust mRNA regulation in yeast.
  • Overlapping yet distinct mechanisms of these PUF proteins allow for nuanced gene expression control.
  • This dual-protein regulatory strategy may be a common mechanism in 3'-UTR-mediated gene control.