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Analysis of RIN1 gene expression in colorectal cancer
Katsunori Senda1, Takanori Goi, Yasuo Hirono
1First Department of Surgery, University of Fukui, Yoshida-Gun, Fukui 910-1193, Japan.
Abstract:
The RIN1 gene, located on chromosome 11q13.2, is a molecule consisting of a coding region of 2352 bp, has a domain on the 3' side that binds to H-Ras protein, and is presumed to be an important molecule in an intracellular signaling pathway. Since the RIN1 molecule belonging to the effector molecules of H-Ras has not been reported in colorectal or other digestive tract cancers to date, we investigated how the RIN1 gene was involved in colorectal cancer. Fifty-two (51.5%) of 101 colorectal cancer specimens strongly expressed the RIN1 gene compared to the adjacent normal colorectal tissue. The 5-year survival rate of patients positive for the expression of the RIN1 gene was significantly poorer at 55% than that (83%) of patients negative for the expression of the RIN1 gene. Also, we confirmed that RIN1 protein was localized chiefly in the cytoplasm of colorectal cancer cell lines, and bound to 14-3-3 protein, but not to Ras protein. These results indicate that the RIN1 gene serves as an important signal transduction system for evaluating the malignancy of colorectal cancer.
Insights
The RIN1 gene is highly expressed in colorectal cancer, correlating with poorer patient survival. This gene may play a role in cancer malignancy and signal transduction.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The RIN1 gene, located on chromosome 11q13.2, is involved in intracellular signaling pathways and binds to H-Ras protein.
- RIN1's role in digestive tract cancers, including colorectal cancer, has not been previously reported.
Purpose of the Study:
- To investigate the involvement of the RIN1 gene in colorectal cancer.
- To determine if RIN1 expression correlates with patient survival and malignancy.
Main Methods:
- Analysis of RIN1 gene expression in 101 colorectal cancer specimens and adjacent normal tissues.
- Assessment of 5-year survival rates based on RIN1 expression.
- Immunocytochemical analysis of RIN1 protein localization and binding partners (14-3-3 and Ras proteins) in colorectal cancer cell lines.
Main Results:
- Strong expression of the RIN1 gene was observed in 51.5% of colorectal cancer specimens compared to normal tissue.
- Patients with positive RIN1 gene expression had a significantly poorer 5-year survival rate (55%) compared to those negative for expression (83%).
- RIN1 protein was primarily found in the cytoplasm of colorectal cancer cells and bound to 14-3-3 protein, not Ras protein.
Conclusions:
- The RIN1 gene is frequently overexpressed in colorectal cancer.
- RIN1 expression is a significant indicator of poor prognosis in colorectal cancer patients.
- RIN1 may function as a crucial component of the signal transduction system, influencing colorectal cancer malignancy.