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Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene) Layers on Gold Microelectrodes for Uric Acid-Sensing Applications
Published on: July 28, 2021
Process development for the electroenzymatic synthesis of (R)-methylphenylsulfoxide by use of a 3-dimensional
S Lütz1, K Vuorilehto, A Liese
1Institute of Biotechnology 2, Research Centre Jülich GmbH, D-52425 Jülich, Germany. s.luetz@fz-juelich.de
Abstract:
The electrogeneration of hydrogen peroxide via reduction of dissolved oxygen was carried out in a three-dimensional electrochemical cell using graphite grains as cathode material and combined with the enantioselective oxidation of thioanisole to (R)-methylphenylsulfoxide catalyzed by Chloroperoxidase from Caldariomyces fumago. The relevant parameters for process development (e.g., conductivity of the biotransformation medium) were identified and optimized, leading to an efficient electroenzymatic process with a space-time yield of 104 g x L(-1) x d(-1). The isolated product was produced on gram scale (1.2 g, purity >98%, ee > 98.5%).
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