Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reply: Stem cell-based embryo models: scientific promise clashes with ethical reality.

Human reproduction (Oxford, England)·2026

Same author

The development and impact of an innovative graduate program to promote entry to physiology-related careers: the Master of Health Science in Medical Physiology.

Advances in physiology education·2026

Same author

Human stem cell-based embryo models: innovation, ethics, and policy.

Human reproduction (Oxford, England)·2026

Same author

A Phase 1 Prognostic Trial for Predicting Paediatric Allergy Using the Placenta at Birth.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2026

Same author

Fire ant rafts offer principles and rules for synthetic programmable morphing matter.

Bioinspiration & biomimetics·2026

Same author

Enhancing rigor and reproducibility: Implementing STAR Methods and the ISSCR Standards Checklist at Stem Cell Reports.

Stem cell reports·2026

Search research articles

Related Experiment Video

Updated: Jul 16, 2026

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Published on: July 25, 2016

Trophoblast-specific gene manipulation using lentivirus-based vectors.

Pantelis Georgiades¹, Brian Cox, Marina Gertsenstein

¹Developmental Genetics and Embryology Research Unit, Department of Biological Sciences, University of Cyprus, Nicosia, Cyprus. pgeor@ucy.ac.cy

|March 30, 2007

Summary

Researchers developed a new lentivirus tool for precise gene manipulation in mouse placental trophoblast cells. This system aids in understanding gene functions essential for embryonic development and survival.

More Related Videos

Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs

Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs

Published on: November 1, 2018

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Published on: March 16, 2017

Related Experiment Videos

Last Updated: Jul 16, 2026

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Published on: July 25, 2016

Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs

Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs

Published on: November 1, 2018

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Published on: March 16, 2017

Area of Science:

Developmental Biology
Genetics
Reproductive Biology

Background:

The mammalian placenta, formed by trophoblast cells, is crucial for embryonic development and survival.
Genetic studies in mice are often complicated by placental failure, hindering interpretation of knockout phenotypes.
Specific tools for manipulating gene expression in trophoblast lineages are needed to clarify gene functions.

Purpose of the Study:

To develop and validate a lentivirus-mediated system for efficient and specific gene manipulation in mouse trophoblast progenitor cells.
To enable both gain-of-function and loss-of-function studies in the trophoblast lineage during placental development.

Main Methods:

Lentivirus-mediated delivery of transgenes into mouse blastocysts.
Expression of green fluorescent protein (GFP) to track trophoblast progenitor labeling.
Utilizing short hairpin RNA (shRNA) delivered by lentivirus for gene knockdown.
Employing Cre recombinase delivered by lentivirus for gene knockout.

Main Results:

Successful rapid and specific expression of GFP in outer trophoblast progenitors and their placental derivatives.
Efficient trophoblast-specific gene knockdown achieved using pol III-driven shRNA lentiviral vectors.
Efficient trophoblast-specific gene knockout demonstrated using pol II-driven Cre recombinase lentiviral vectors.

Conclusions:

A novel lentivirus-based system enables lineage-specific gene manipulation in mouse trophoblast cells.
This system facilitates the study of gene roles in placental development and embryonic viability.
The approach holds potential for application in other mammalian species for placental research.