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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Tissue Transplantation01:24

Tissue Transplantation

Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...
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Transgenic Organisms

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Transgenic Organisms00:53

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Related Experiment Video

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Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
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Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Published on: October 1, 2012

Conditional gene expression: a new tool for the transplantologist.

J S Maltzman1, L A Turka

  • 1Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA. maltz@mail.med.upenn.edu

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
|March 30, 2007
PubMed
Summary

Genetically modified mice enable studying complex biological processes. Conditional gene targeting offers precise control over gene function, overcoming limitations of traditional methods for better research outcomes.

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Area of Science:

  • Genetics and Genomics
  • Developmental Biology
  • Immunobiology

Background:

  • Genetically engineered mice are crucial tools in biological research.
  • Conventional gene targeting often results in embryonic lethality, hindering tissue-specific studies.
  • Conditional gene expression allows for controlled gene manipulation, enabling normal development before targeted gene deletion.

Purpose of the Study:

  • To review advanced techniques for conditional gene targeting in mice.
  • To highlight the advantages of temporal and tissue-specific gene manipulation.
  • To focus on the Cre-loxP and tetracycline systems for inducible gene expression.

Main Methods:

  • Review of conditional gene targeting strategies in mice.
  • Focus on the Cre-loxP recombination system.
  • Discussion of drug-inducible gene expression using the tetracycline system.

Main Results:

  • Conditional gene targeting overcomes limitations of conventional methods.
  • Cre-loxP system allows for precise genetic modifications.
  • Tetracycline system enables temporal control over gene expression.

Conclusions:

  • Conditional gene targeting in mice significantly advances biological research.
  • These techniques provide powerful tools for studying gene function in specific tissues and at specific times.
  • Continued development of these methods promises deeper insights into development, cell biology, and disease.