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Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
Published on: January 3, 2020
Inhibitory effects of various plant polyphenols on the toxicity of Staphylococcal alpha-toxin
Oksun Choi1, Kinnosuke Yahiro, Naoko Morinaga
1Department of Molecular Infectiology, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo ward, Chiba 260-8670, Japan.
Abstract:
Staphylococcal alpha-toxin, known for its wide spectrum of biological activities, is involved in the pathogenesis of Staphylococcal infectious diseases. In recent years, various phytochemicals have been found to have antimicrobiological, including antibacterial, antiviral and antifungal, and antitoxic activities. We investigated whether several plant polyphenols inhibit alpha-toxin activities in vitro and in vivo. We found hop bract tannin (HBT) and apple condensed tannin (ACT) to exert inhibitory effects on alpha-toxin cytotoxicity. HBT also reduced the murine skin inflammatory effect and the lethality of alpha-toxin. These polyphenols formed aggregates with alpha-toxin and thereby inhibited its activities. Inhibition of alpha-toxin by HBT and ACT was dose dependent, suggesting that these polyphenols may be a useful adjunct to current treatments for alpha-toxin catalyzed Staphylococcal infectious diseases.
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