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Related Experiment Video

Updated: Jul 16, 2026

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
08:43

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Published on: February 14, 2017

Working hypothesis to redefine endothelial progenitor cells.

D N Prater1, J Case, D A Ingram

  • 1Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, USA.

Leukemia
|March 30, 2007
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Summary

Endothelial progenitor cells (EPCs) from bone marrow were thought to drive new blood vessel growth. This review examines the controversial methods used to define and quantify EPCs, focusing on cell culture techniques.

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Published on: April 13, 2012

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Last Updated: Jul 16, 2026

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
08:43

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

Published on: February 14, 2017

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood
07:26

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood

Published on: September 14, 2017

Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood
13:46

Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood

Published on: April 13, 2012

Area of Science:

  • Cardiovascular Research
  • Cell Biology
  • Regenerative Medicine

Background:

  • Postnatal vasculogenesis via bone marrow (BM)-derived endothelial progenitor cells (EPCs) has been proposed as a key mechanism for neoangiogenesis since 1997.
  • EPCs have been investigated as biomarkers for cardiovascular disease severity and as a potential cell-based therapy for cardiovascular disorders.

Purpose of the Study:

  • To review and define endothelial progenitor cells (EPCs) based on widely cited published reports.
  • To provide a framework for understanding subsequent research in the rapidly evolving field of EPCs.
  • To focus specifically on studies utilizing in vitro cell culture techniques for EPC definition.

Main Methods:

  • Review of published literature focusing on endothelial progenitor cells (EPCs).
  • Analysis of studies employing in vitro cell culture techniques for EPC identification and enumeration.
  • Examination of methodologies used in widely cited reports to define EPCs.

Main Results:

  • The definition and quantification of EPCs have been primarily based on two controversial methodologies: in vitro cell culture and flow cytometry.
  • This review specifically synthesizes findings from studies that used cell culture techniques to define EPCs.

Conclusions:

  • The definition of endothelial progenitor cells (EPCs) remains a subject of debate, impacting their utility as biomarkers and therapeutic agents.
  • A clear understanding of EPC definition, particularly through cell culture methods, is crucial for advancing research in cardiovascular disease and regenerative medicine.