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Related Experiment Video

Updated: Jul 16, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Imaging subcellular scattering contrast by using combined optical coherence and multiphoton microscopy.

Shuo Tang¹, Chung-Ho Sun, Tatiana B Krasieva

¹Department of Electrical and Computer Engineering, University of British Columbia, Vancouver V6T 1Z4, Canada.

|March 30, 2007

Summary

This study visualizes subcellular structures using light scattering microscopy. Researchers identified scattering contrast from mitochondria, membranes, and filaments within single cells.

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Area of Science:

Biophysics
Cell Biology
Optical Microscopy

Background:

Understanding subcellular structure origins of light scattering is crucial for advanced imaging.
Coherent reflectance imaging offers potential for label-free visualization of cellular components.

Purpose of the Study:

To investigate the structural basis of scattering contrast in single cells.
To correlate scattering signals with specific subcellular components using a combined microscopy approach.

Main Methods:

Utilized a combined optical coherence and multiphoton microscope system.
Employed a 12 fs Ti:sapphire laser source and a 0.95 numerical aperture objective.
Coregistered high-resolution coherence-gated scattering images with two-photon-excited fluorescence images.

Main Results:

Observed significant scattering contrast from mitochondria, plasma membrane, and actin filaments.
Identified scattering originating from the cytoplasm-nucleus boundary.
Found minimal scattering contribution from the nuclear core.

Conclusions:

Confirmed that light scattering signals can visualize specific subcellular structures.
Demonstrated the utility of coherent reflectance geometry for label-free cellular imaging.
Highlighted the potential of scattering contrast for identifying key cellular components.