High-resolution surface-plasmon imaging in air and in water: V(z) curve and operating conditions

We present what are believed to be the first images obtained with a far-field high-resolution scanning surface-plasmon microscope in an aqueous medium. Measurements of V(z), the output response of the microscope, versus defocus z give a signature of the surface-plasmon propagation. V(z) is strongly conditioned by the laser beam diameter and the objective's numerical aperture, and we show how the operating mode (in air and in water) must be chosen to maximize the surface-plasmon field and to minimize diffraction (edge) effects.