A link between monoamine oxidase-A and apoptosis in serum deprived human SH-SY5Y neuroblastoma cells
J C Fitzgerald1, C Ufer, E E Billett
1School of Biomedical and Natural Sciences, Nottingham Trent University, Nottingham, UK.
Abstract:
Increased monoamine oxidase (MAO) activity was recently shown to accompany apoptotic cell death of various neuronal cells following growth factor deprivation. Here we show that in serum deprived SH-SY5Y cells, MAO-A mRNA levels and catalytic activities are increased, linked with activation of the apoptotic executioner caspase-3. Importantly, specific inhibition of MAO-A activity resulted in loss of apoptotic cell morphology. Our study indicates that MAO catalytic activity is involved in apoptotic signalling in response to serum withdrawal in neuronal cells.
Insights
Increased monoamine oxidase-A (MAO-A) activity in neuronal cells contributes to programmed cell death following serum deprivation. Inhibiting MAO-A activity prevented the development of apoptotic cell morphology, highlighting its role in cell death signaling.
Area of Science:
- Neuroscience
- Cell Biology
- Biochemistry
Background:
- Increased monoamine oxidase (MAO) activity is linked to neuronal cell death after growth factor deprivation.
- The specific role of MAO in apoptosis signaling remains to be fully elucidated.
Purpose of the Study:
- To investigate the role of monoamine oxidase-A (MAO-A) in serum deprivation-induced apoptosis in SH-SY5Y neuronal cells.
- To determine if MAO-A activity is a critical factor in the apoptotic pathway.
Main Methods:
- Serum deprivation of SH-SY5Y cells.
- Measurement of MAO-A mRNA levels and catalytic activity.
- Assessment of caspase-3 activation.
- Inhibition of MAO-A activity using specific inhibitors.
- Evaluation of apoptotic cell morphology.
Main Results:
- Serum deprivation led to increased MAO-A mRNA levels and catalytic activity in SH-SY5Y cells.
- MAO-A activity correlated with the activation of the apoptotic executioner caspase-3.
- Specific inhibition of MAO-A activity significantly reduced the characteristic apoptotic cell morphology.
Conclusions:
- MAO-A catalytic activity plays a crucial role in the apoptotic signaling pathway triggered by serum withdrawal in neuronal cells.
- MAO-A is a potential therapeutic target for conditions involving neuronal cell death.
More Related Videos
19:44Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen
Published on: May 30, 2012
08:07Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
Published on: February 17, 2016
