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Updated: Jul 16, 2026

Exploring Protein-Glycan Interactions: Advances in Nuclear Magnetic Resonance
Published on: August 26, 2025
Analysis of glycans in glycoproteins by diffusion-ordered nuclear magnetic resonance spectroscopy
Karin Ortner1, Veeramuthu N Sivanandam, Wolfgang Buchberger
1Institute of Analytical Chemistry, Johannes Kepler University, Altenberger Strasse 69, 4040, Linz, Austria.
Abstract:
Enzymatically cleaved glycans from sub-milligram quantities of erythropoietin (EPO) and ovalbumin have been analyzed, without further purification, by two-dimensional diffusion-ordered nuclear magnetic resonance spectroscopy. At NMR sample concentrations below 50 micromol L-1 the major components of the oligosaccharide fractions could be distinguished by their anomeric proton chemical shift and their size-dependent diffusion coefficients.
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