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Updated: Jun 21, 2026
Use of Human Perivascular Stem Cells for Bone Regeneration
Published on: May 25, 2012
[Pilot study of using autologous bone marrow stromal cells and coral to repair canine segmental mandibular defects]
Jie Yuan1, Guang-peng Liu, Gang Chai
1Department of Plastic and Reconstructive Surgery, 9th People's Hospital affiliated to Shanghai Jiao Tong University Medical School, Shanghai Key Laboratory of Tissue Engineering, Shanghai 200011, China.
Objective:
To repair segmental mandibular defects with autologous bone marrow stromal cells (BMSCs) engineered bone.
Methods:
Isolated BMSCs were expanded in vitro and osteogenic induced. In 12 canines, a 3 cm segmental mandibular defect at right mandible was created. 6 canine's defects were repaired with cell-scaffold constructs made from induced BMSCs and coral; others were repaired with coral as control. The engineered bone was evaluated by X-ray, CT, Dual Energy X-ray Absorptiometry (DXA), gross and histological examination, and biomechanical test post-operatively.
Results:
Induced BMSCs grew well on coral scaffold. At 12 weeks, X-ray showed more callus formed in experimental group, while evident scaffold duration in control group. At 32 weeks, gross observation, X-ray and CT demonstrated well bony-union in experimental group, while bony-nonunion in control group. Also DXA revealed significantly higher bone mineral density of experimental group than control group. Histologically, mature bone were commonly observed and there were bony healing in experimental group, while fibrous healing occurred in control group. Biomechanical test revealed no significant difference between experimental group and normal group.
Conclusions:
Canine segmental mandibular defects can be repaired with the tissue-engineered bone generated by coral scaffold with autologous osteogenic BMSCs.
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