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Related Concept Videos

Cross-reactivity00:42

Cross-reactivity

Overview
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.

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Immune reactivity of candidate reference materials.

Montserrat Fernandez-Rivas1, Marja Aalbers, Kay Fötisch

  • 1Servicio de Allergía, Hospital Clinio San Carlos, Madrid.

Arbeiten Aus Dem Paul-Ehrlich-Institut (Bundesamt Fur Sera Und Impfstoffe) Zu Frankfurt A.M
|March 31, 2007
PubMed
Summary

Recombinant allergens were evaluated for immune reactivity as potential reference materials. Some recombinant allergens, like Bet v 1 and Der p 2, closely mimic natural versions, while others, such as rPhl p 1, show reduced potency.

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Area of Science:

  • Immunology
  • Allergen standardization
  • Biotechnology

Background:

  • Assessing the quality of recombinant allergens is crucial for their use as reference materials.
  • Immune reactivity differences between natural and recombinant allergens impact diagnostic accuracy.

Purpose of the Study:

  • To analyze and compare the immune reactivity of natural and recombinant major allergens from birch pollen, grass pollen, olive pollen, and house dust mites.
  • To evaluate the suitability of recombinant allergens as potential reference materials.

Main Methods:

  • Utilized direct Radioallergosorbent Test (RAST) and RAST inhibition to assess IgE binding capacity.
  • Measured biological activity through basophil histamine release assays.
  • Employed sera from allergic patients across Europe for testing.

Main Results:

  • Recombinant Bet v 1 (birch pollen) demonstrated excellent mimicry of natural allergen IgE binding.
  • Recombinant Phl p 1 (grass pollen) showed significantly lower IgE reactivity, while rPhl p 5a exhibited closer immune reactivity to its natural form.
  • Recombinant Ole e 1 (olive pollen) had lower IgE binding but higher histamine release activity.
  • Recombinant group 1 house dust mite allergens were less potent, but group 2 allergens were close mimics of natural counterparts.

Conclusions:

  • Recombinant allergens exhibit variable immune reactivity compared to their natural counterparts.
  • Specific recombinant allergens, such as rBet v 1 and Der p 2, show promise as reference materials due to their high similarity in immune response.
  • Further evaluation is needed for allergens like rPhl p 1 and rOle e 1 to determine their suitability as reference standards.