Related Experiment Video
Updated: Jul 16, 2026
Detection and Isolation of Campylobacter spp. from Raw Meat
Published on: February 23, 2024
Detection of Campylobacter jejuni and Campylobacter coli in chicken meat samples by real-time nucleic acid
E Churruca1, C Girbau, I Martínez
1Departamento de Inmunología, Microbiología y Parasitología, Facultad de Farmacia, Universidad del País Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain.
Abstract:
A nucleic acid sequence-based amplification (NASBA) assay based on molecular beacons was used for real-time detection of Campylobacter jejuni and Campylobacter coli in samples of chicken meat. A set of specific primers and beacon probe were designed to target the 16S rRNA of both species. The real-time NASBA protocol including the RNA isolation was valid for both of the cell suspensions in buffered saline and the artificially contaminated chicken meat samples. The presence of rRNA could be correlated with cellular viability, following inactivation of the bacteria by heating, in inoculated chicken meat samples but not in RNase-free cell suspensions.
