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Molecularly imprinted poly beta-cyclodextrin polymer: application in protein refolding.

Mohammad Ali Esmaeili1, Razieh Yazdanparast

  • 1Institute of Biochemistry and Biophysics, P.O. Box 13145-1384, University of Tehran, Tehran, Iran.

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A novel molecularly imprinted polymer effectively strips 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) detergent from alkaline phosphatase. This method enhances protein refolding yield and prevents aggregation, offering a cost-effective industrial alternative.

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Area of Science:

  • Biochemistry
  • Polymer Science
  • Protein Chemistry

Background:

  • Refolding alkaline phosphatase with 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) showed low recovery yields.
  • The bulky nature of CHAPS interferes with stripping agents like cyclodextrin, hindering efficient protein refolding.
  • Detergent stripping is crucial for successful protein refolding processes.

Purpose of the Study:

  • To investigate the potential of a molecularly imprinted polymer as a novel stripping agent for CHAPS.
  • To improve the efficiency of CHAPS stripping and enhance alkaline phosphatase refolding yields.
  • To evaluate the use of a molecularly imprinted poly(beta-CD) polymer for protein refolding.

Main Methods:

  • Utilized a molecularly imprinted polymer with CHAPS as the template.
  • Studied the refolding of GuHCl denatured alkaline phosphatase.
  • Optimized the refolding environment for the new stripping agent.

Main Results:

  • Achieved a 79% refolding yield for denatured alkaline phosphatase using 20 mg/ml of the molecularly imprinted poly(beta-CD) polymer.
  • The new stripping agent demonstrated high potential in preventing protein aggregate formation.
  • The polymer offers advantages such as recyclability and ease of separation.

Conclusions:

  • The molecularly imprinted polymer is an effective alternative to soluble beta-cyclodextrin for CHAPS stripping.
  • This new strategy offers a promising approach for protein refolding at reduced industrial costs.
  • The polymer's ability to prevent aggregation makes it ideal for industrial protein refolding applications.