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Related Concept Videos

Radical Autoxidation01:20

Radical Autoxidation

The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...

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Related Experiment Video

Updated: Jul 16, 2026

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
10:30

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

Published on: June 10, 2022

Antioxidant measurements.

Anikó Somogyi1, Klára Rosta, Péter Pusztai

  • 12nd Department of Internal Medicine, Semmelweis University, Budapest, Hungary. somogyi@be12.sote.hu

Physiological Measurement
|March 31, 2007
PubMed
Summary

Free radicals cause cellular damage, potentially leading to disease. This review summarizes methods for measuring the antioxidant defense system and total antioxidant capacity to combat oxidative stress.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Medicine

Background:

  • Cellular chemical reactions, including oxidation and reduction, generate free radicals.
  • Free radicals cause oxidative damage to lipids, proteins, and DNA, potentially contributing to diseases.
  • The body's antioxidant system, comprising enzymatic and non-enzymatic antioxidants, defends against this oxidative stress.

Purpose of the Study:

  • To review general aspects of methods for measuring the total antioxidant capacity.
  • To discuss current methods for detecting antioxidant properties.

Main Methods:

  • Literature review of established and emerging antioxidant measurement techniques.
  • Analysis of methodologies for assessing the comprehensive antioxidant defense system.

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  • Evaluation of assays for quantifying specific antioxidant activities.

    • Main Results:

    • Oxidative stress from free radicals is a significant factor in cellular dysfunction and disease.
    • A robust antioxidant defense system is crucial for cellular protection.
    • Various methods exist to quantify total antioxidant capacity and individual antioxidant properties.

    Conclusions:

    • Understanding and measuring the antioxidant system is vital for assessing health and disease risk.
    • Accurate measurement of antioxidant capacity aids in the development of therapeutic strategies.
    • This review provides a comprehensive overview of antioxidant detection methods for researchers.