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Reactive oxygen species plasmatic levels in ischemic stroke.
Laura Nanetti1, Ruja Taffi, Arianna Vignini
1Institute of Biochemistry, Università Politecnica delle Marche, Ancona, Italy.
Molecular and Cellular Biochemistry
|March 31, 2007
Summary
Oxidative stress and increased peroxynitrite (ONOO-) levels are linked to acute ischemic stroke. This study found decreased nitric oxide (NO) and higher inducible NO synthase (iNOS) in stroke patients, suggesting their role in brain injury.
Area of Science:
- Neuroscience
- Biochemistry
- Pathology
Background:
- Oxidative stress contributes to neuronal damage following ischemia-reperfusion.
- Plasma antioxidant activity may protect against stroke-associated neurological damage.
Purpose of the Study:
- Investigate oxidative stress, nitric oxide (NO), and peroxynitrite (ONOO-) levels in acute ischemic stroke patients.
- Examine inducible nitric oxide synthase (iNOS), endothelial nitric oxide synthase (eNOS), and nitrotyrosine expression in these patients.
Main Methods:
- Compared NO and ONOO- levels in patients with atherothrombotic/lacunar stroke versus controls.
- Assessed iNOS, eNOS, and nitrotyrosine protein expression using densitometric analysis.
Main Results:
- Stroke patients showed significantly higher plasma ONOO- and lower NO levels compared to controls.
- Patients exhibited significantly higher iNOS and nitrotyrosine (N-Tyr) protein levels.
- A decrease in NO is likely due to increased iNOS expression following thrombotic events.
Conclusions:
- Free radical production and oxidative stress play a significant role in ischemic brain injury pathogenesis.
- Elevated peroxynitrite (ONOO-) may serve as a key marker for brain damage and neurological impairment in acute ischemic stroke.
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