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Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

Small interfering RNAs (siRNA)

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

piRNA - Piwi-interacting RNAs

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

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Related Experiment Video

Updated: Jul 16, 2026

RNAi Interference by dsRNA Injection into Drosophila Embryos

RNAi Interference by dsRNA Injection into Drosophila Embryos

Published on: April 11, 2011

Development of strategies for conditional RNA interference.

Danny Allen¹, Paul F Kenna, Arpad Palfi

¹Department of Genetics, Trinity College Dublin, Dublin 2, Ireland. danny.allen@tcd.ie

The Journal of Gene Medicine

|April 3, 2007

Summary

This study introduces a new method for tissue-specific gene silencing using RNA interference (RNAi). The novel constructs generate potent small interfering RNA (siRNA) while minimizing potential off-target effects for enhanced RNAi technology.

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RNAi Interference by dsRNA Injection into Drosophila Embryos

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Published on: April 11, 2011

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

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Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Published on: May 4, 2018

Area of Science:

Molecular Biology
Gene Expression Regulation

Background:

RNA interference (RNAi) enables sequence-specific gene suppression using double-stranded RNA (dsRNA).
Traditional RNAi often uses polymerase III promoters for ubiquitous expression, which can be problematic for tissue-specific applications.
Ensuring dsRNA potency requires minimal 5' and 3' overhangs, posing challenges for polymerase II promoters.

Purpose of the Study:

To develop a method for generating potent, tissue-specific RNA interference (RNAi).
To overcome limitations of using polymerase II promoters for controlled dsRNA expression.

Main Methods:

Utilized polymerase II promoters combined with cis-acting hammerhead ribozymes and short-hairpin RNA sequences.
Constructs were designed to generate potent dsRNA molecules within specific tissues.

Main Results:

Novel constructs successfully produced functional small interfering RNA (siRNA).
Demonstrated suppression of enhanced green fluorescent protein (eGFP) both in vitro and in vivo (in mice).
Observed no significant type-1 interferon response compared to traditional H1-transcribed short hairpin RNA (shRNA).

Conclusions:

The developed system allows for tissue-specific expression of dsRNA, reducing potential off-target effects.
This approach enhances the resolution and applicability of RNAi technologies.
The system is adaptable for precise control of gene silencing in targeted tissues.