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Related Experiment Videos

TRH-related peptides in the rabbit prostate complex during development.

C R Thetford1, J M Morrell, S M Cockle

  • 1Department of Biochemistry and Physiology, University of Reading, U.K.

Biochimica Et Biophysica Acta
|January 23, 1992
PubMed
Summary

Rabbit prostate tissue contains novel TRH-related peptides, pyroglutamylglutamylprolineamide (pGlu-Glu-ProNH2) and a polypeptide fragment. Levels increase with age, primarily localized to the prostate gland.

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Area of Science:

  • Endocrinology
  • Peptide Chemistry
  • Reproductive Biology

Background:

  • A novel tripeptide, pyroglutamylglutamylprolineamide (pGlu-Glu-ProNH2), and a TRH-immunoreactive polypeptide fragment have been identified in the rabbit prostate complex.
  • These peptides are found in high concentrations in the prostate and semen.

Purpose of the Study:

  • To investigate age-related changes in the levels of TRH-related peptides in the rabbit prostate complex.
  • To determine the specific location of these peptides within the prostate complex.

Main Methods:

  • Peptide extraction from rabbit prostate complexes across various age groups (11 weeks to 2 years).
  • Separation using gel exclusion chromatography and identification via TRH radioimmunoassay.
  • Anion and cation exchange chromatography to characterize the TRH-immunoreactive components.

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  • Regional dissection of the prostate complex followed by extraction and analysis.

    • Main Results:

    • TRH-immunoreactive peptide concentrations were low at 11 weeks but significant in older rabbits.
    • The majority of low molecular weight TRH immunoreactivity co-eluted with synthetic pGlu-Glu-ProNH2.
    • Non-authentic TRH was identified, as it did not bind to cation exchange chromatography.
    • TRH-related peptides were exclusively localized to the prostate region of the complex.

    Conclusions:

    • TRH-related peptides, including pGlu-Glu-ProNH2, accumulate in the rabbit prostate with age.
    • The prostate gland is the primary site for these novel TRH-related peptides.
    • Further research is needed to elucidate the physiological role of these peptides.