Related Experiment Video
Updated: Jul 16, 2026

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
Published on: September 11, 2018
Spectrophotometric determination of aqueous cyanide using a revised phenolphthalin method
David Cacace1, Heidi Ashbaugh, Naomi Kouri
1Department of Chemistry and Physics, University of North Florida, Jacksonville, FL 32224, USA.
Abstract:
This paper focuses on a revision of the phenolphthalin method for cyanide analysis, with the intent of producing a robust and sensitive spectrophotometric method. Limitations of the phenolphthalin chemistry were overcome by the addition of EDTA to the reagent. The revised reagent was found to have suitably fast kinetics, a linear dynamic range of 0.01-3.0 ppm cyanide, and a detection limit of 5 ppb. The method was tested for interferences and applied to the determination of cyanide in environmental waters.
More Related Videos
Related Concept Videos
Precipitation Titration: Endpoint Detection Methods
In the Volhard method, a standard excess of AgNO3 is first added to the...
Determining the pH of Salt Solutions
Redox Titration: Iodimetry and Iodometry
Redox Titration: Overview
Indicators
Precipitation Titration: Overview
A precipitation titration curve demonstrates the change in concentration of the titrant or analyte upon adding the...

