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Updated: Jul 15, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
Published on: May 9, 2020
Monitoring aminoglycoside-induced conformational changes in 16S rRNA through acrylamide quenching
Pei-Wen Chao1, Christine S Chow
1Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202, USA.
Abstract:
Fluorescence of 2-aminopurine (2AP)-substituted A-site and acrylamide quenching were used to study the interactions of paromomycin and neamine with the decoding region of 16S rRNA. The results reveal that paromomycin binding to the A-site RNA leads to increased exposure of residue A1492. In contrast, neamine has little effect on the solvent accessibility of A1492. Electrospray ionization mass spectrometry was used to compare the affinity of paromomycin with the A-site and 2-AP-substituted A-site RNAs.

