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Updated: Jul 15, 2026

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
Published on: May 5, 2014
A high-throughput screening platform for inhibitors of the riboflavin biosynthesis pathway
Johannes Kaiser1, Boris Illarionov, Felix Rohdich
1Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, D-85748 München, Germany.
Abstract:
3,4-Dihydroxy-2-butanone 4-phosphate synthase, 6,7-dimethyl-8-ribityllumazine synthase, and riboflavin synthase of the riboflavin biosynthetic pathway are potential targets for novel antiinfective drugs. This article describes a platform for high-throughput screening for inhibitors of these enzymes. The assays can be monitored photometrically and have been shown to be robust, as indicated by Z factors 0.87. A (13)C NMR assay for hit verification of 3,4-dihydroxy-2-butanone 4-phosphate synthase inhibitors is also reported.
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