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Published on: July 21, 2018
Reproducibility of 18F-FDG microPET studies in mouse tumor xenografts
Mangal Dandekar1, Jeffrey R Tseng, Sanjiv S Gambhir
1Department of Radiology and the Bio-X Program, Stanford University School of Medicine, Stanford, California, USA.
Unlabelled:
(18)F-FDG has been used to image mouse xenograft models with small-animal PET for therapy response. However, the reproducibility of serial scans has not been determined. The purpose of this study was to determine the reproducibility of (18)F-FDG small-animal PET studies.
Methods:
Mouse tumor xenografts were formed with B16F10 murine melanoma cells. A 7-min small-animal PET scan was performed 1 h after a 3.7- to 7.4-MBq (18)F-FDG injection via the tail vein. A second small-animal PET scan was performed 6 h later after reinjection of (18)F-FDG. Twenty-five sets of studies were performed. Mean injected dose per gram (%ID/g) values were calculated from tumor regions of interest. The coefficient of variation (COV) from studies performed on the same day was calculated to determine the reproducibility. Activity from the second scans performed after 6 h were adjusted by subtracting the estimated residual activity from the first (18)F-FDG injection. For 7 datasets, an additional scan immediately before the second injection was performed, and residual activity from this additional delayed scan was subtracted from the activity of the second injection. COVs of both subtraction methods were compared. Blood glucose values were measured at the time of injection and used to correct the %ID/g values.
Results:
The COV for the mean %ID/g between (18)F-FDG small-animal PET scans performed on the same day 6 h apart was 15.4% +/- 12.6%. The delayed scan subtraction method did not produce any significant change in the COV. Blood glucose correction increased the COV. The injected dose, tumor size, and body weight did not appear to contribute to the variability of the scans.
Conclusion:
(18)F-FDG small-animal PET mouse xenograft studies were reproducible with moderately low variability. Therefore, serial small-animal PET studies may be performed with reasonable accuracy to measure tumor response to therapy.
Insights
Serial (18)F-FDG small-animal PET scans in mouse xenografts show moderate reproducibility. This indicates potential for accurate therapy response assessment in preclinical cancer research.
Area of Science:
- Preclinical imaging
- Oncology research
- Nuclear medicine
Background:
- (18)F-FDG PET is used for therapy response assessment in mouse xenografts.
- Reproducibility of serial scans is crucial but undetermined.
Purpose of the Study:
- Determine the reproducibility of serial (18)F-FDG small-animal PET studies in mouse xenografts.
Main Methods:
- 25 sets of serial (18)F-FDG PET scans were performed on mouse xenografts.
- Scans were 6 hours apart; COV calculated for reproducibility.
- Blood glucose corrected %ID/g values.
Main Results:
- Mean %ID/g COV was 15.4% +/- 12.6% between scans.
- Delayed scan subtraction and blood glucose correction did not improve reproducibility.
- Injected dose, tumor size, and body weight did not affect variability.
Conclusions:
- (18)F-FDG small-animal PET studies in mouse xenografts are reproducible with moderate variability.
- Serial scans can be used with reasonable accuracy for therapy response evaluation.
![Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F62460.jpg&w=3840&q=50)
