Related Experiment Video
Updated: Jul 15, 2026
Laser-scanning Photostimulation of Optogenetically Targeted Forebrain Circuits
Published on: December 27, 2013
Manipulation of early region 4
1Department of Genetic Medicine, Weill Medical College of Cornell University, New York, NY, USA.
Abstract:
Adenovirus early region 4 (E4) regulates processes in infected cells that include viral late gene expression, nonhomologous end joining, responses to DNA damage, and apoptosis. E4 is essential for viral growth in most cell lines. In this chapter, the current knowledge of the functions of six E4 products is summarized briefly. Protocols are presented for manipulation of E4, incorporation of E4 mutations into the viral genome, and growth of E4 mutants on complementing cell lines. A compilation of the described E4-complementing cell lines is included.
Insights
Adenovirus early region 4 (E4) is crucial for viral replication, controlling gene expression and DNA repair. This chapter details E4 functions and methods for studying E4 mutants using specialized cell lines.
Area of Science:
- Molecular Virology
- Cellular Biology
- Genetics
Background:
- Adenovirus early region 4 (E4) plays a vital role in regulating cellular processes during viral infection.
- Key functions include control of viral late gene expression, DNA repair mechanisms, and apoptosis.
- E4 is indispensable for efficient viral replication in most host cell types.
Purpose of the Study:
- To summarize current knowledge on the functions of six Adenovirus E4 proteins.
- To provide protocols for manipulating the E4 region and generating E4 mutants.
- To compile a list of E4-complementing cell lines for mutant propagation.
Main Methods:
- Review of existing literature on Adenovirus E4 functions.
- Description of genetic manipulation techniques for the E4 region.
- Methodologies for growing E4 mutants on complementing cell lines.
Main Results:
- A comprehensive overview of the diverse roles of E4 products in viral infection.
- Detailed protocols for creating and culturing viral strains with modified E4 regions.
- An updated compilation of cell lines that can support the growth of E4-deficient adenoviruses.
Conclusions:
- Adenovirus E4 is a multifaceted regulator essential for viral propagation.
- The provided methods facilitate further research into E4 functions and viral pathogenesis.
- Availability of complementing cell lines aids in the study of E4-mutant viruses.
