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Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method
R Daniel Gietz1, Robert H Schiestl
1Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.
Nature Protocols
|April 3, 2007
Abstract:
Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2-3 h, once the yeast cells have been grown depending on the heat shock used.
