Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bioreactor Controls-III

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mannosylated hemagglutinin peptides bind cyanovirin-N independent of disulfide-bonds in complementary binding sites.

RSC advances·2022

Same author

Particle Radiation Side-Effects: Intestinal Microbiota Composition Shapes Interferon-γ-Induced Osteo-Immunogenicity.

Radiation research·2022

Same author

Cyanovirin-N Binds Viral Envelope Proteins at the Low-Affinity Carbohydrate Binding Site without Direct Virus Neutralization Ability.

Molecules (Basel, Switzerland)·2021

Same author

Intestinal bacterial indicator phylotypes associate with impaired DNA double-stranded break sensors but augmented skeletal bone micro-structure.

Carcinogenesis·2019

Same author

A novel probiotic, Lactobacillus johnsonii 456, resists acid and can persist in the human gut beyond the initial ingestion period.

Gut microbes·2018

Same author

Retraction Note to: Mouse models of intestinal inflammation and cancer.

Archives of toxicology·2018

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method.

R Daniel Gietz¹, Robert H Schiestl

¹Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.

Nature Protocols

|April 3, 2007

Summary

This study presents a rapid yeast transformation protocol for screening complex plasmid libraries. The lithium acetate method efficiently transforms Saccharomyces cerevisiae for applications like yeast two-hybrid analysis.

More Related Videos

High-throughput Yeast Plasmid Overexpression Screen

High-throughput Yeast Plasmid Overexpression Screen

Published on: July 27, 2011

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Published on: February 21, 2025

Related Experiment Videos

Last Updated: Jul 15, 2026

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

High-throughput Yeast Plasmid Overexpression Screen

High-throughput Yeast Plasmid Overexpression Screen

Published on: July 27, 2011

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Published on: February 21, 2025

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

High-throughput screening of genetic libraries is crucial for biological discovery.
Efficient transformation protocols are essential for yeast-based genetic studies.
Complex plasmid libraries require robust transformation methods.

Purpose of the Study:

To describe a streamlined transformation protocol for Saccharomyces cerevisiae.
To provide a method suitable for screening complex plasmid libraries.
To optimize transformation for yeast two-hybrid analysis.

Main Methods:

Utilized the lithium acetate/single-stranded carrier DNA/polyethylene glycol (PEG) transformation method.
Applied the protocol to Saccharomyces cerevisiae.
Focused on optimizing the transformation for library screening.

Main Results:

The described protocol offers a transformation efficiency suitable for library screening.
The procedure is time-efficient, completing within 2.5 hours post-culture growth.
Demonstrated suitability for complex plasmid libraries.

Conclusions:

The lithium acetate/ssDNA/PEG method is an effective and rapid protocol for yeast transformation.
This protocol facilitates efficient screening of complex plasmid libraries in Saccharomyces cerevisiae.
The method is particularly valuable for yeast two-hybrid screening applications.