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Related Concept Videos

Thin-Layer Chromatography (TLC): Overview01:11

Thin-Layer Chromatography (TLC): Overview

Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...

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Related Experiment Video

Updated: Jul 15, 2026

Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique
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Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique

Published on: October 6, 2022

[Quantitative TLC analysis of Valepotriates.]

G Rücker1, M Neugebauer, M S El Din

  • 1Pharmazeutisches Institut der Rheinischen Friedrich Wilhelms-Universität, Bonn, Bundesrepublik Deutschland.

Planta Medica
|November 1, 1981
PubMed
Summary

No abstract available in PubMed .

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