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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Related Experiment Video

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Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
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Gene expression profiling in equine muscle tissues using mouse cDNA microarrays.

E Mucher1, L Jayr, F Rossignol

  • 1INRA, Laboratoire d'Etude de la Physiologie de l'Exercice, Genopole, Evry, France.

Equine Veterinary Journal. Supplement
|April 4, 2007
PubMed
Summary

Mouse cDNA microarrays can analyze gene expression in equine muscle, offering insights into exercise adaptations and disorders. This study confirms the feasibility and sensitivity of using these arrays for equine muscle RNA, enabling exploration of muscle metabolism changes.

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Area of Science:

  • Molecular Biology
  • Equine Genetics
  • Exercise Physiology

Background:

  • Understanding metabolic adaptations in equine muscle is crucial for addressing exercise-related disorders.
  • Microarray technology offers a potential avenue for analyzing gene expression in equine muscle.
  • Previous research has not fully explored the feasibility of using cross-species microarrays for equine gene expression profiling.

Purpose of the Study:

  • To evaluate the feasibility of employing mouse complementary DNA (cDNA) microarrays for analyzing gene expression profiles in normal equine muscles.
  • To assess the interspecific hybridization efficiency and reproducibility of mouse cDNA microarrays with equine RNA.
  • To identify differentially expressed genes between equine gluteus medius and longissimus lumborum muscles.

Main Methods:

  • Equine muscle biopsies were obtained from healthy Standardbred horses.
  • Total RNA was extracted, amplified, and its quality assessed.
  • Gene expression profiling was performed using mouse cDNA microarrays (15,264 unique genes) with dye-swap hybridizations and signal filtering.

Main Results:

  • Mouse cDNA microarrays demonstrated successful hybridization with equine muscle cDNA, confirmed by positive control gene amplification.
  • Reproducibility tests showed high linearity (99.99% of significant genes within a 1.4-fold change threshold).
  • Comparison between gluteus medius and longissimus lumborum revealed 63 upregulated and 8 downregulated genes, with functional classification performed.

Conclusions:

  • Mouse microarrays are a feasible tool for hybridizing equine muscle RNA, owing to significant sequence identities.
  • The method is sensitive enough to quantify gene expression changes after applying appropriate filters.
  • Gene expression profiling in horses can be utilized to investigate muscle metabolism alterations linked to exercise, training, pathology, and medication.