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Updated: Jul 15, 2026

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
Published on: September 20, 2016
Prostaglandin E2 synthase in syncytiotrophoblastic vesicles found in the placental intervillous space
1Department of Pathology, Keio University Hospital, Tokyo, Japan. tofu@bea.hi-ho.ne.jp
Objective:
The purpose of this study was to confirm microsomal prostaglandin E2 synthase (mPGES) in the vesicles found in the intervillous space.
Study Design:
Immunohistochemical staining for mPGES was conducted to confirm this enzyme in vesicles by using fresh sections of mature placentas.
Results:
Staining for mPGES was prominent in vesicles separating from syncytiotrophoblast into the intervillous space. Syncytiotrophoblast was partly positive or negative for mPGES. Vesicles showed a tendency to degenerate with a weakly positive or negative reaction for mPGES. Extravillous cytotrophoblast was strongly positive for mPGES in the basal plate.
Conclusion:
The vesicles containing mPGES suggest the formation of prostaglandin E2 (PGE2). This enzyme could be concentrated in vesicles by a different secretory mechanism.
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