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Rps4 maps near the inactivation center on the mouse X chromosome
1Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, London, United Kingdom.
Genomics
|February 1, 1992
Summary
Researchers mapped the mouse Rps4 gene, a homolog of human RPS4X, to the X chromosome near the X-inactivation center. This finding helps understand gene order conservation between mouse and human X chromosomes.
Area of Science:
- Genetics
- Molecular Biology
- Genomics
Background:
- Ribosomal protein S4 (RPS4) has Y-linked (RPS4Y) and X-linked (RPS4X) human homologs.
- The human RPS4X locus is near the X-inactivation center but escapes inactivation.
Purpose of the Study:
- To isolate and map the mouse Rps4 locus, the homolog of human RPS4X.
- To investigate the conservation of gene order on the X chromosome between humans and mice.
Main Methods:
- Isolation of a mouse Rps4 genomic clone.
- Development of an intron-specific hybridization probe.
- Genetic mapping using interspecific mouse backcrosses.
Main Results:
- The mouse Rps4 gene was mapped to the X chromosome, near the Phka locus and the X-inactivation center.
- The gene order Ccg-1-Rps4/Phka-Xist-Pgk-1 was found to be conserved between mouse and human X chromosomes.
Conclusions:
- The mouse Rps4 locus is homologous to human RPS4X and located near the X-inactivation center.
- The conserved gene order suggests shared evolutionary mechanisms on the X chromosome.