Related Experiment Video

Updated: Jul 15, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

The Notch1/c-Myc pathway in T cell leukemia

Vishva Mitra Sharma1, Kyle M Draheim, Michelle A Kelliher

  • 1Department of Cancer Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.

Cell Cycle (Georgetown, Tex.)
|April 4, 2007
PubMed

Insights

The Notch1 pathway directly regulates c-Myc, driving cancer growth. This Notch1-c-Myc signaling is crucial for leukemia and mammary tumor development, offering therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Notch signaling is frequently deregulated in human cancers.
  • The oncogenic roles of Notch1 and c-Myc are well-established.
  • A direct link between Notch1 and c-Myc was previously uncharacterized.

Purpose of the Study:

  • To investigate the direct transcriptional relationship between Notch1 and c-Myc.
  • To determine the role of the Notch1-c-Myc pathway in cancer cell growth and survival.
  • To provide evidence for the contribution of Notch1-c-Myc signaling in tumorigenesis.

Main Methods:

  • Identification of c-Myc as a direct transcriptional target of Notch1 in T cell acute lymphoblastic leukemia (T-ALL).
  • Analysis of leukemic cell lines to assess dependence on the Notch1-c-Myc pathway.
  • Genetic studies in mouse models of mammary tumorigenesis.

Main Results:

  • c-Myc was identified as a direct transcriptional target of the Notch1 pathway.
  • Leukemic cell growth and survival in mouse tal1 tumor models were dependent on the Notch1-c-Myc pathway.
  • Genetic evidence confirmed the Notch1-c-Myc pathway's contribution to mouse mammary tumorigenesis.

Conclusions:

  • Notch1 directly regulates c-Myc expression.
  • The Notch1-c-Myc pathway is essential for sustaining leukemic cell growth and survival.
  • Notch1 contributes to T cell and epithelial cell transformation, at least in part, by maintaining c-Myc levels.

More Related Videos

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity07:09

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...