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09:00

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Published on: May 22, 2012

Improvement of the sensitivity and resolution of PCR-SSCP analysis with optimized primer concentrations in PCR

Xilin Zhu1, Nifang Niu, Yang Liu

  • 1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, China.

Journal of Genetics
|April 5, 2007
PubMed
Abstract

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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