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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jul 15, 2026

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
07:55

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

High-throughput knockout screen in fission yeast.

Juraj Gregan1, Peter K Rabitsch, Cornelia Rumpf

  • 1Research Institute of Molecular Pathology, Dr Bohr-Gasse 7, 1030 Vienna, Austria. juraj.gregan@univie.ac.at

Nature Protocols
|April 5, 2007
PubMed
Summary

We developed an efficient, large-scale gene knockout strategy for fission yeast (Schizosaccharomyces pombe). This high-throughput method enables the deletion of 96 genes simultaneously, accelerating genetic research.

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A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
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Published on: March 7, 2019

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Area of Science:

  • Genetics
  • Molecular Biology
  • Yeast Biology

Background:

  • Large-scale gene knockout is crucial for functional genomics.
  • Previous methods for Schizosaccharomyces pombe gene deletion were not optimized for high-throughput screening.

Purpose of the Study:

  • To develop an efficient, large-scale gene knockout strategy for fission yeast (Schizosaccharomyces pombe).
  • To create a searchable database of knockout constructs for all predicted fission yeast genes.
  • To validate the technique by identifying novel genes involved in chromosome segregation.

Main Methods:

  • Utilized knockout constructs with homologous regions to target genes and dominant drug-resistance markers.
  • Implemented a 96-well format for simultaneous deletion of multiple genes.
  • Developed a searchable online database for accessing knockout constructs.

Main Results:

  • Established a highly efficient, large-scale gene knockout protocol for Schizosaccharomyces pombe.
  • Created a comprehensive database of knockout constructs for all predicted fission yeast genes.
  • Identified novel genes essential for chromosome segregation during meiosis.

Conclusions:

  • The developed protocol significantly enhances the efficiency of large-scale gene knockout in fission yeast.
  • The accessible database facilitates further research into gene function.
  • This method accelerates the discovery of genes involved in critical biological processes like chromosome segregation.