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A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
Published on: October 1, 2009
Use of a commercially available reagent for the selective detection of homocysteine in plasma
Jorge O Escobedo1, Weihua Wang, Robert M Strongin
1Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
Nature Protocols
|April 5, 2007
Abstract:
A procedure for the detection of homocysteine (Hcy) in blood plasma is described. A commercially available chromogen is added to the plasma sample. The plasma solution turns from yellow to blue upon heating for 4 min when a detectable threshold level of Hcy is present. Chromatographic separations and immunogenic materials are not needed. The protocol takes approximately 30 min.
