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Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Related Experiment Video

Updated: Jul 15, 2026

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

A protocol for Agrobacterium-mediated transformation in rice.

Asuka Nishimura¹, Ikuko Aichi, Makoto Matsuoka

¹Honda Research Institute Japan Co. Ltd, 2-1-4 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan. asuka.nishimura@jp.honda-ri.com

Nature Protocols

|April 5, 2007

Summary

This study presents a new Agrobacterium-mediated rice transformation method. It achieves high transformation frequencies (30-50%) in both easily cultured and recalcitrant elite japonica rice varieties within 2-3 months.

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Last Updated: Jul 15, 2026

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A Simplified Method for Agrobacterium-mediated Transformation of Phytophthora palmivora

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Published on: March 20, 2026

Area of Science:

Plant Biotechnology
Genetics and Molecular Biology
Agricultural Science

Background:

Agrobacterium-mediated transformation is crucial for rice functional genomics.
Current methods are limited to japonica rice varieties with high regeneration potential.
Elite japonica and most indica rice varieties exhibit low transformation efficiencies due to regeneration difficulties.

Purpose of the Study:

To develop an improved Agrobacterium-mediated rice transformation protocol.
To enhance transformation efficiency across diverse rice genotypes, including recalcitrant varieties.
To facilitate high-throughput functional analysis in a broader range of rice.

Main Methods:

Utilized an optimized Agrobacterium-mediated transformation approach.
Applied the method to both easily cultured and elite japonica rice varieties.
Focused on improving regeneration and transformation efficiency.

Main Results:

Achieved successful Agrobacterium-mediated transformation in elite japonica rice.
Obtained transgenic rice plants within a 2-3 month timeframe.
Reported transformation frequencies ranging from 30-50% for both easily cultured and recalcitrant varieties.

Conclusions:

The developed method broadens the applicability of Agrobacterium-mediated rice transformation.
This protocol overcomes regeneration limitations in elite japonica rice.
Enables efficient genetic modification for functional studies in diverse rice germplasms.