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Related Experiment Video

Updated: Jul 15, 2026

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Published on: June 7, 2013

A simple, robust and semi-automated parasite egg isolation protocol.

Ted H M Mes¹, Maarten Eysker, Harm W Ploeger

¹Netherlands Institute of Ecology (NIOO-KNAW), Centre for Estuarine and Marine Ecology, POB 140, 4400AC, Yerseke, The Netherlands. t.mes@nioo.knaw.nl

Nature Protocols

|April 5, 2007

Summary

This study introduces a novel protocol for large-scale nematode egg quantification from fecal samples. The method enhances efficiency for epidemiological and genetic studies of host-parasite interactions.

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Area of Science:

Veterinary Parasitology
Molecular Epidemiology
Host-Parasite Interactions

Background:

Accurate parasite quantification is crucial for understanding host-parasite dynamics.
Traditional methods like the McMaster technique are limited in sample size and standardization for large-scale studies.

Purpose of the Study:

To develop and describe a standardized protocol for high-throughput nematode egg quantification.
To facilitate large-scale epidemiological and genetic analyses of host-parasite interactions.

Main Methods:

A protocol utilizing low-density salt solution for flotation and centrifugation of nematode eggs.
Subsequent sucrose flotation and precipitation for clear egg preparations.
Analysis of up to 6g of feces per sample, with preparations suited for digital recording and automated counting.

Related Experiment Videos

Last Updated: Jul 15, 2026

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Published on: June 7, 2013

Main Results:

The protocol yields clear, concentrated egg preparations in microtiter plates.
Processing time is approximately 40 minutes for 24 samples, from fecal suspension to digital recording.
Enables standardized processing of large sample volumes, overcoming limitations of traditional methods.

Conclusions:

The described protocol offers an efficient and scalable solution for parasite quantification.
It supports advanced analyses in host-parasite epidemiology and genetics through digital data acquisition.