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Updated: Jul 15, 2026
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Published on: March 11, 2014
[Detection and typification of human papilloma virus in pre cancerous cervical lesions]
Susana Aedo A1, Angélica Melo A, Patricia García
1Departamento de Anatomía Patológica, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile.
Background:
The association of different genotypes of human papilloma virus (HPV) with cervical cancer is well known. However, there is little information about their association with pre-cancerous lesions.
Aim:
To assess the frequency of different HPV genotypes in pre cancerous cervical lesions.
Material And Methods:
A cervical sample was obtained by cytobrush in 15 women with low grade lesions and 40 women with high grade lesions, subjected to conization by loop electrical excision procedure (LEEP). Detection and typification of HPV was done by polymerase chain reaction and restriction fragment length polymorphism.
Results:
All women were infected with HPV. Eighty five percent of samples were typified. A unique HPV subtype was found in 76% of women. Fourteen percent had an infection with multiple subtypes and in 10%, the viral genotype was not identified. The most common subtypes found were HPV 16, HPV 52 and HPV 53.
Conclusions:
There is a high rate of infection with HPV with a high oncogenic risk among these women.
