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Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

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Related Experiment Video

Updated: Jul 15, 2026

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
12:36

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Published on: January 14, 2016

Methyl-CpG-binding domain (MBD) proteins in plants.

Gideon Grafi1, Assaf Zemach, Letizia Pitto

  • 1Albert Katz Department of Dryland Biotechnologies, Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion 84990, Israel. ggrafi@bgu.ac.il

Biochimica Et Biophysica Acta
|April 5, 2007
PubMed
Summary

Plant DNA methylation, a key epigenetic process, is interpreted by methyl-CpG-binding domain (MBD) proteins. These MBD proteins are crucial for regulating gene expression and chromatin structure in plants.

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Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
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Published on: January 28, 2011

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Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
09:23

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm

Published on: January 28, 2011

Area of Science:

  • Plant molecular biology
  • Epigenetics
  • Genomics

Background:

  • Cytosine methylation is the primary epigenetic modification in plant nuclear DNA, affecting symmetrical (CpG, CpNpG) and asymmetrical contexts.
  • Cytosine methylation plays a critical role in genome organization, gene expression, and overall plant growth and development.
  • Understanding how the methyl group is functionally interpreted has been a recent focus, leading to the identification of methyl-5-cytosine binding proteins.

Purpose of the Study:

  • To review recent advancements in plant methyl-CpG-binding domain (MBD) proteins.
  • To explore the potential roles of plant MBD proteins in mediating chromatin structure through CpG methylation.

Main Methods:

  • Isolation and characterization of methylated DNA-binding proteins.
  • Review of existing literature on plant MBD proteins and their functions.
  • Analysis of the evolutionary conservation of MBD proteins.

Main Results:

  • Methyl-CpG-binding domain (MBD) proteins, conserved across species, bind to 5-methylcytosine.
  • These proteins are key mediators in interpreting the functional state of DNA methylation.
  • Plant MBD proteins are implicated in controlling chromatin structure.

Conclusions:

  • Plant MBD proteins are essential components of the epigenetic machinery.
  • Further research into plant MBD proteins will elucidate their precise roles in chromatin regulation and development.
  • The study highlights the significance of MBD proteins in linking DNA methylation to functional outcomes in plants.