Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Islet hormone pulse intervals are dependent upon sampling frequency.

J Stagner1, A Nakagawa, A Peiris

  • 1Research Service, Veterans Administration Medical Center, Louisville, KY 40206.

Life Sciences
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Randomized, double-blind, placebo-controlled study vs data in the daily practice using linaclotide in patients with irritable bowel syndrome with constipation.

Neurogastroenterology and motility·2018
Same author

Determining an optimal dose of linaclotide for use in Japanese patients with irritable bowel syndrome with constipation: A phase II randomized, double-blind, placebo-controlled study.

Neurogastroenterology and motility·2017
Same author

Role of lipid modification on a starch-debranching enzyme, Klebsieila pullulanase: comparison of properties of lipid-modified and unmodified pullulanases.

Molecular microbiology·2017
Same author

Autonomic function and control of pancreatic somatostatin.

Diabetologia·2016
Same author

A laser-induced pulsed water jet for layer-selective submucosal dissection of the esophagus.

Laser therapy·2016
Same author

Clinical Features and Outcomes of Diffuse Alveolar Hemorrhage During Antithrombotic Therapy: A Retrospective Cohort Study.

Lung·2016
Same journal

MAGED1 stabilizes NEUROD1 to promote Per3 expression in the pineal gland.

Life sciences·2026
Same journal

TNF-centered network pharmacology and molecular modeling of selected Andrographis paniculata compounds in hypertension.

Life sciences·2026
Same journal

Retraction notice to "Beneficial effect of Calculus Bovis Sativus on 17α-ethynylestradiol-induced cholestasis in the rat" [Life Sci. 113 (2014) 22-30].

Life sciences·2026
Same journal

Soluble PD-1 drives renal fibrosis in CKD by disrupting immune homeostasis: Therapeutic mitigation via a targeted sPD-1 sequestration strategy.

Life sciences·2026
Same journal

METTL1 promotes hepatic steatosis by mediating m<sup>7</sup>G modification of ALOX15B mRNA.

Life sciences·2026
Same journal

SLC30A9-mediated mitochondrial zinc homeostasis drives osteosarcoma chemoresistance by suppressing the mtDNA-cGAS-STING pathway.

Life sciences·2026
See all related articles

The frequency of sampling significantly impacts the calculated pulse interval for pulsatile insulin secretion. Higher sampling rates are crucial for accurately determining insulin pulse periods, avoiding spurious results.

Area of Science:

  • Endocrinology
  • Physiology
  • Biostatistics

Background:

  • Pulsatile insulin secretion is a known physiological process observed in various experimental systems.
  • Discrepancies exist regarding the basal frequency and interpulse intervals of insulin secretion, both in vivo and in vitro.

Purpose of the Study:

  • To investigate the hypothesis that sampling frequency profoundly affects the calculation of insulin pulse intervals.
  • To determine the optimal sampling frequency for accurate assessment of islet hormone secretory pulses.

Main Methods:

  • Analysis of artificial data with a known 11-minute pulse period.
  • Measurement of insulin concentrations from canine pancreatic perfusates sampled at 1-minute intervals.
  • Assessment of human peripheral blood insulin levels sampled at 2-minute and 5-minute intervals.

Related Experiment Videos

  • Application of Pulsar and Cycle Detector algorithms across diverse sampling intervals (1-60 minutes).

    • Main Results:

    • Sampling frequencies exceeding 2 minutes can generate spurious pulse trains with altered periods for insulin secretory pulses.
    • Calculated pulse intervals varied significantly depending on the sampling frequency used for data acquisition.
    • Artificial data confirmed that sampling intervals longer than the true pulse period can lead to misinterpretation.

    Conclusions:

    • The accuracy of islet hormone secretory pulse period calculations is critically dependent on the sampling frequency.
    • High-frequency sampling (e.g., every 1-2 minutes) is recommended for reliable analysis of pulsatile insulin secretion.
    • Future studies must carefully consider and report sampling frequencies to ensure reproducibility and accurate interpretation of insulin secretion dynamics.