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Related Experiment Videos

A sensitive fluorometric assay for reducing sugars.

F Chen1, Y Liu, J Lu

  • 1Department of Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles 90033.

Life Sciences
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

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A new fluorometric assay accurately detects reducing sugars at nanomolar levels. This rapid method simplifies sugar quantification without complex separation steps.

Area of Science:

  • Analytical Chemistry
  • Biochemistry

Background:

  • Accurate quantification of reducing sugars is crucial in various biological and chemical applications.
  • Existing fluorometric assays may require complex procedures like chromatographic separation.

Purpose of the Study:

  • To develop a simple, rapid, and sensitive fluorometric assay for reducing sugars.
  • To offer an alternative to existing methods that avoids chromatographic separation.

Main Methods:

  • Derivatization of reducing sugars with hydrazine at pH 3 to form hydrazones.
  • Reaction of hydrazones with fluorescamine at pH 9.4 and 7.4.
  • Quantification of sugars via fluorescence intensity measurement (Ex: 400 nm, Em: 490 nm).

Main Results:

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  • The assay demonstrates high sensitivity, detecting sugars in the nanomolar range.
  • It exhibits precision and reproducibility with intra- and inter-run variations below 4%.
  • The assay can also quantify aliphatic and aromatic aldehydes, but not acetone.

    • Conclusions:

    • A novel, efficient fluorometric assay for reducing sugars has been established.
    • This method offers advantages in simplicity and speed compared to existing techniques.
    • Potential applications include adaptation for HPLC postcolumn detection of sugars and aldehydes.