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Effect of Xenopus laevis oocyte extract on supercoiled simian virus 40 DNA: formation of complex DNA
Abstract:
A soluble cell-free extract derived from stage 6 Xenopus laevis oocytes is described. From supercoiled simian virus 40 DNA the extract produces nicked circles (having a single-strand scission), linear molecules of full unit size, shorter length fragments, and various forms of complex DNA.